Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Cdk2 antibody cell signaling.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Recommended fluorochrome conjugated anti rabbit secondary antibodies.
To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.
Merged red and green signal.
Mix well then add 0 1 g bsa mix.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Western blot analysis was performed using cdk12 antibody.
The antibody weakly cross reacts with cdc2 phosphorylated at thr161.
Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Lanes 1 3 and 5.
Find out why customers rank cst highest for antibody specificity and sensitivity.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.
It does not cross react with other phosphorylated cyclin dependent kinases.
Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells.
Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160.
Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Wild type hap1 cell lysate 20 µg lanes 2 4 and 6.
Kinase activity associated with cyclin a cdc2 is found only in g2 phase.
Lane 1 is 10 input.
Green signal from target ab32147 observed at 34 kda lanes 3 and 4.
An antibody shouldn t be one of the variables in your experiment.
