The idexx bioanalytics cell line authentication guide has the answers.
Cell line authentication kit.
Geneprint 10 coamplifies and detects nine human loci th01 tpox vwa amelogenin csf1po d16s539 d7s820 d13s317 d5s818 and d21s11 these loci collectively provide a genetic profile with a random match.
Standardization of str profiling guidelines using multiple kits.
This is the same technology used by the fbi for human dna identification testing hall explains.
The profiling kits are only suitable for cell lines of human origin.
Cell line authentication kits cross contaminated or misidentified cell lines can ruin experiments leading to loss of precious time and funding.
The test utilizes primers that pick up repeated segments of dna that are typically 2 6 base pairs in length.
Mouse cell line authentication possible contamination is detected by one mouse gender marker two human markers and a 12 plex panel including 9 mouse tetranucleotide microsatellites.
Grcf dna services offers cell line authentication analysis following ansi atcc asn 0002 2011 authentication of human cell lines.
The geneprint 10 system provides a convenient str based method for cell line identity confirmation and for detection of intraspecies contamination in cultured human cell lines.
The current recommended method for cell line authentication is short tandem repeat str profiling.
The impact of cell line contamination.
The number of repeats at each.
Str profiling aids in the detection of misidentified cross contaminated or genetically drifted cells which invalidate research results.
Human str profiling cell authentication service short tandem repeat profiling of cell lines is an easy to use way to authenticate confirm the identity of your cells.
Briefly gdna was purified from cell pellets using the invitrogen recoverall total nucleic acid isolation kit for formalin fixed paraffin embedded ffpe samples.
Within the past five years a handful of publications have been retracted due to invalidated use of certain cell lines.
To show the utility of the cla identifiler plus kit and seqstudio instrument for cell line authentication we analyzed dna from cultures of five different commonly used cell lines.
Using standardized techniques authentication enables communication among all users about the resource and ensures valid reproducible experimental results.
Rapd assay design for the authentication of mammalian microbial and other cell lines has to be species specific.
Authentication of a cell line is the sum of the process by which a line s identity is verified and shown to be free of contamination from other cell lines and microbes.
