Cell Sorting Flow Cytometry

Researchers partnering with nyu langone s cytometry and cell sorting laboratory have access to the most powerful and adaptable flow cytometry and cell sorting technologies and instruments available.

Cell sorting flow cytometry.

Download transcript pdf slide 1. There are two methods for performing cell sorting. With flow cytometry cell sorting multiple detectors are being utilized to analyze different cell characteristics. The amount of refraction refers to the size and volume of the cell.

Cell sorting by flow cytometry cell sorting is a method to purify cell populations based on the presence or absence of specific physical characteristics. Using flow cytometry requires a flow cytometric cell sorter like the bd facsaria. Download slides pdf transcript. Cells larger than lymphocytes require a larger nozzle to be placed on the instrument and the flow rate is lower.

One is by using flow cytometry and the other is by magnetically labeling the cells to differentiate and separate the cell populations. Michael timm s pearl on cell sorting using flow cytometry explains the origins of cell sorting technology the two main types and their differences as well as best practices and troubleshooting sorting assays. For example if lymphocytes were concentrated to 20 million per ml the flow rate at the instrument could be run as high as 20 000 cells per second. Hello my name is michael timm.

Forward scatter fsc forward light scatter refers to the refracted light past the cell into a detector behind the laser.